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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Trafficking ER Proteins

Anti-ERp57 antibody [4F9] (ab223363)

Anti-ERp57 antibody [4F9] (ab223363)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [4F9] to ERp57
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-ERp57 antibody [4F9]
    See all ERp57 primary antibodies
  • Description

    Mouse monoclonal [4F9] to ERp57
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human ERp57 aa 25-505. Full length mature His tagged chain.
    Database link: P30101

  • Positive control

    • WB: HeLa cell lysate. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    4F9
  • Isotype

    IgG2b
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [4F9] (ab223363)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [4F9] (ab223363)

    4% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ERp57 (green) using ab223363 at 1/200 dilution in ICC/IF. The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red) at 1/1000 dilution. The lower right panel shows the merged images.

  • Western blot - Anti-ERp57 antibody [4F9] (ab223363)
    Western blot - Anti-ERp57 antibody [4F9] (ab223363)
    Anti-ERp57 antibody [4F9] (ab223363) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 56.8 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    Block: 5% skimmed milk in TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ERp57 antibody [4F9] (ab223363)

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  • FITC Anti-ERp57 antibody [Map.ERP57] (ab183396)

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    Anti-ERp57 antibody (ab13507)

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    Anti-ERp57 antibody [EPR10678(B)] (ab154191)

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