Anti-ERp57 antibody [4F9] (ab223363)
Key features and details
- Mouse monoclonal [4F9] to ERp57
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-ERp57 antibody [4F9]
See all ERp57 primary antibodies -
Description
Mouse monoclonal [4F9] to ERp57 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human ERp57 aa 25-505. Full length mature His tagged chain.
Database link: P30101 -
Positive control
- WB: HeLa cell lysate. ICC/IF: HeLa cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4F9 -
Isotype
IgG2b -
Research areas
Images
-
4% Formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ERp57 (green) using ab223363 at 1/200 dilution in ICC/IF. The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red) at 1/1000 dilution. The lower right panel shows the merged images.
-
Anti-ERp57 antibody [4F9] (ab223363) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 15 µg
Secondary
Goat Anti-Mouse IgG HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 56.8 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesBlock: 5% skimmed milk in TBST.