Anti-ERp57 antibody (ab88098)
Key features and details
- Rabbit polyclonal to ERp57
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-ERp57 antibody
See all ERp57 primary antibodies -
Description
Rabbit polyclonal to ERp57 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88098 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications SheepGoatCowBaboonOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).Target
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Tissue specificity
Detected in the flagellum and head region of spermatozoa (at protein level). -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 100689343 Chinese hamster
- Entrez Gene: 281803 Cow
- Entrez Gene: 2923 Human
- Entrez Gene: 14827 Mouse
- Entrez Gene: 100174305 Orangutan
- Omim: 602046 Human
- SwissProt: P38657 Cow
- SwissProt: P30101 Human
see all -
Alternative names
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
see all
Images
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Western blot - Anti-ERp57 antibody (ab88098)All lanes : Anti-ERp57 antibody (ab88098) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PDIA3 (ERp57) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDaLanes 1 - 2: Merged signal (red and green). Green - ab88098 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab88098 was shown to recognize PDIA3 in wild-type HAP1 cells as signal was lost at the expected MW in PDIA3 (ERp57) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDIA3 (ERp57) knockout samples were subjected to SDS-PAGE. Ab88098 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-ERp57 antibody (ab88098)All lanes : Anti-ERp57 antibody (ab88098) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 5 : CHO-K1 cell lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab88098 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ERp57 antibody (ab88098)All lanes : Anti-ERp57 antibody (ab88098) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PDIA3 (ERp57) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 57 kDaLanes 1 - 2: Merged signal (red and green). Green - ab88098 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab88098 was shown to recognize PDIA3 in wild-type HAP1 cells as signal was lost at the expected MW in PDIA3 (ERp57) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PDIA3 (ERp57) knockout samples were subjected to SDS-PAGE. Ab88098 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-ERp57 antibody (ab88098)All lanes : Anti-ERp57 antibody (ab88098) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lane 5 : CHO-K1 cell lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
