Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [CL2452] to ERp57
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG2b

Product name

Anti-ERp57 antibody [CL2452]
See all ERp57 primary antibodies
Description

Mouse monoclonal [CL2452] to ERp57
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment corresponding to Human ERp57 aa 100-250.
Database link: P30101

Run BLAST with Run BLAST with
Positive control
- IHC-P: Human small intestine, placenta, testis, skin and fallopian tube tissue. WB: U-251 MG whole cell lysate.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

CL2452
Isotype

IgG2b
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2452] (ab242362)

Paraffin-embedded human small intestine tissue stained for ERp57 using ab242362 at 1/5000 dilution in immunohistochemical analysis. Strong cytoplasmic immunoreactivity in glandular epithelium and lamina propria cells is observed.
Western blot - Anti-ERp57 antibody [CL2452] (ab242362)

Anti-ERp57 antibody [CL2452] (ab242362) at 1 µg/ml + U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2452] (ab242362)

Paraffin-embedded human fallopian tube tissue stained for ERp57 using ab242362 at 1/5000 dilution in immunohistochemical analysis. Strong cytoplasmic immunoreactivity in glandular epithelium cells is observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2452] (ab242362)

Paraffin-embedded human skin tissue stained for ERp57 using ab242362 at 1/5000 dilution in immunohistochemical analysis. Moderate cytoplasmic positivity in epithelial cells is observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2452] (ab242362)

Paraffin-embedded human testis tissue stained for ERp57 using ab242362 at 1/5000 dilution in immunohistochemical analysis. Strong cytoplasmic positivity in semiferous tubules is observed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2452] (ab242362)

Negative control:

Paraffin-embedded human skeletal muscle tissue stained for ERp57 using ab242362 at 1/5000 dilution in immunohistochemical analysis. Shows absence of immunoreactivity (negative control).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2452] (ab242362)

Paraffin-embedded human placenta tissue stained for ERp57 using ab242362 at 1/5000 dilution in immunohistochemical analysis. Strong cytoplasmic positivity in trophoblast is observed.

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