Anti-PDIA6 antibody (ab11432)
Key features and details
- Rabbit polyclonal to PDIA6
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PDIA6 antibody
See all PDIA6 primary antibodies -
Description
Rabbit polyclonal to PDIA6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Hamster, Xenopus laevis, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human PDIA6 aa 476-492.
Sequence:DIDLSDVELDDLGKDEL
(Peptide available asab11432)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDIA6 antibody (ab11432)
Western blot of PDIA6 in HT 1080 cell lysate using ab11432.
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Immunocytochemistry/ Immunofluorescence - Anti-PDIA6 antibody (ab11432)ICC/IF image of ab11432 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
