Anti-ERp29 antibody (ab11420)
Key features and details
- Rabbit polyclonal to ERp29
- Suitable for: IP, Flow Cyt, IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Non human primates
- Isotype: IgG
Overview
-
Product name
Anti-ERp29 antibody
See all ERp29 primary antibodies -
Description
Rabbit polyclonal to ERp29 -
Host species
Rabbit -
Tested applications
Suitable for: IP, Flow Cyt, IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Non human primates -
Immunogen
Recombinant full length protein (His-tag) corresponding to Rat ERp29.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ERp29 antibody (ab11420)All lanes : Anti-ERp29 antibody (ab11420) at 1 µg/ml
Lane 1 : MDCK (canine kidney cell line) membrane-enriched extract
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) membrane-enriched extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) membrane-enriched extract
Lane 4 : A549 (human lung carcinoma cell line) membrane-enriched extract
Lane 5 : MCF7 (human breast adenocarcinoma cell line) membrane-enriched extract
Lane 6 : A431 (human epidermoid carcinoma cell line) membrane-enriched extract
Lane 7 : HEK-293 (human epithelial cell line from embryonic kidney) membrane-enriched extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa12% SDS-PAGE
-
Western blot - Anti-ERp29 antibody (ab11420)Western blot of ERp29 in HEK cell lysate using ab11420. -
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)ICC/IF image of ab11420 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11420, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labeling ERp29 with ab11420.
-
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of NS-1 cells labeling ERp29 with ab11420.
-
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of p19 cells labeling ERp29 with ab11420.
-
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of rat thyrocytes labeling ERp29 with ab11420.
