Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ERp29 antibody [EPR12499(B)] (ab176573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12499(B)] to ERp29
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ERp29 antibody [EPR12499(B)]
    See all ERp29 primary antibodies

  • Description

    Rabbit monoclonal [EPR12499(B)] to ERp29

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ERp29 aa 50-150 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: P30040

  • Positive control

    • WB: HEK293T, HeLa, HepG2, MCF7 and OVCAR-3 cell lysates. ICC/IF: HeLa cells. IHC-P: Human kidney and liver tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12499(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ERP29 knockout HEK293T cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab176573 observed at 29 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab176573 Anti-ERp29 antibody [EPR12499(B)] was shown to specifically react with ERp29 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266543 (knockout cell lysate ab257188) was used. Wild-type and ERp29 knockout samples were subjected to SDS-PAGE. ab176573 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Western blot - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    All lanes : Anti-ERp29 antibody [EPR12499(B)] (ab176573) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : OVCAR-3 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ERp29 using ab176573 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp29 antibody [EPR12499(B)] (ab176573)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ERp29 using ab176573 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody [EPR12499(B)] (ab176573)

    Immunofluorescent analysis of HeLa cells, labeling ERp29 using ab176573 at a 1/00 dilution (red). DAPI nuclear staining (blue).

  • Anti-ERp29 antibody [EPR12499(B)] (ab176573)
    Anti-ERp29 antibody [EPR12499(B)] (ab176573)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ERp29 antibody [EPR12499(B)] (ab176573)

© 2021 Astana Biomed Group LLP. All rights reserved.