Anti-ERP27 antibody [EPR12503] - BSA and Azide free (ab250398)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12503] to ERP27 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ERP27 antibody [EPR12503] - BSA and Azide free
See all ERP27 primary antibodies -
Description
Rabbit monoclonal [EPR12503] to ERP27 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250398 is the carrier-free version of ab181172.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12503 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab181172, the same antibody clone in a different buffer formulation.Immunofluorescent staining of 4% paraformaldehyde fixed BxPC-3 cells, labeling ERP27 using ab181172 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at a 1/200 dilution and counterstained with Dapi.
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This data was developed using ab181172, the same antibody clone in a different buffer formulation.Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling ERP27 using ab181172 at a 1/50 dilution (green) or Rabbit monoclonal IgG (red), using a Goat anti rabbit IgG (FITC) secondary antibody at a 1/150 dilution.
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This data was developed using ab181172, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling ERP27 using ab181172 at a 1/250 dilution, using a HRP Polymer for Rabbit IgG secondary antibody and counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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All lanes : Anti-C1orf163 antibody [EPR13779-15] (ab181176) at 1/1000 dilution
Lane 1 : Rat pancreas
Lane 2 : Mouse pancreas
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase Conjugated at 1/1000 dilution
Predicted band size: 30 kDaThis data was developed using ab181172, the same antibody clone in a different buffer formulation.
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