Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6563] to Glutathione Synthetase - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free
    See all Glutathione Synthetase primary antibodies

  • Description

    Rabbit monoclonal [EPR6563] to Glutathione Synthetase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HeLa, Daudi and HT-1080 cell lysates; Human colon tissue

  • General notes

    Ab236062 is the carrier-free version of ab133592. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236062 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    Western blot - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    All lanes : Anti-Glutathione Synthetase antibody [EPR6563] (ab133592) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : GSS knockout HEK-293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab133592).

    Lanes 1- 4: Merged signal (red and green). Green - ab133592 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab133592 was shown to react with GSS in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266342 (knockout cell lysate ab257460) was used. Wild-type HEK-293T and GSS knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133592 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    Flow Cytometry - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    Overlay histogram showing Jurkat cells stained with ab133592 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133592, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133592).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Glutathione Synthetase with ab133592 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133592).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    OI-RD Scanning - Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133592).

  • Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)
    Anti-Glutathione Synthetase antibody [EPR6563] - BSA and Azide free (ab236062)

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