Adenosylhomocysteinase (AHCY) Activity Assay Kit (Fluorometric) (ab197002)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
- Sensitivity: 1 µU
Overview
-
Product name
Adenosylhomocysteinase (AHCY) Activity Assay Kit (Fluorometric)
See all SAHH kits -
Detection method
Fluorescent -
Sample type
Cell Lysate, Purified protein, Tissue Lysate -
Assay type
Enzyme activity -
Sensitivity
> 1 µU -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Adenosylhomocysteinase Activity Assay Kit (ab197002) can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant Human AHCY.
-
Notes
Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH) is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Adenosine Standard (10 mM) 1 x 100µl AHCY Assay Buffer 1 x 25ml AHCY Enzyme (Positive Control) 1 x 10µl AHCY Enzyme Mix I 1 vial AHCY Enzyme Mix II 1 vial Homogenization Buffer 1 x 60ml OxiRed Probe in DMSO Red 1 x 200µl SAH Substrate 1 x 100µl -
Research areas
-
Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. -
Pathway
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. -
Involvement in disease
Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. -
Sequence similarities
Belongs to the adenosylhomocysteinase family. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
Alternative names
- Adenosylhomocysteinase
- AdoHcyase
- ahcY
see all
Images
-
Functional Studies - Adenosylhomocysteinase (AHCY) Activity Assay Kit (Fluorometric) (ab197002)
Typical Adenosine Standard Curve obtained following assay protocol.
-
Functional Studies - Adenosylhomocysteinase (AHCY) Activity Assay Kit (Fluorometric) (ab197002)AHCY activity in rat kidney lysate (24 µg), HeLa cell lysate (7.6 µg) and Positive Control; S: Sample, B: Background.
-
Functional Studies - Adenosylhomocysteinase (AHCY) Activity Assay Kit (Fluorometric) (ab197002)AHCY specific activity in HeLa cell lysate (4 µg) and rat kidney lysate (28 µg).
