Adenosylhomocysteinase (AHCY) Inhibitor Assay Kit (Fluorometric) (ab204694)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
Adenosylhomocysteinase (AHCY) Inhibitor Assay Kit (Fluorometric)
See all SAHH kits -
Detection method
Fluorescent -
Sample type
Inhibitor compounds -
Assay type
Enzyme activity -
Product overview
Adenosylhomocysteinase (AHCY) Inhibitor Screning Kit (Fluorometric) (ab204694) is a simple assay to study, screen or characterize potential inhibitors of AHCY. In this assay, the homocysteine generated from the breakdown of S-Adenosyl Homocysteine (SAH) is measured using a Thiol Detecting Reagent which results in enhanced fluorescence that can be measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible, and therefore Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent.
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Notes
Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies).
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 3-Deazaneplanocin A (10 μM) (in DMSO) 1 x 10µl Adenosine Deaminase 1 vial AHCY Assay Buffer 1 x 25ml AHCY Enzyme 1 vial AHCY Reconstitution Buffer 1 x 250µl AHCY Substrate (in DMSO) 1 x 100µl Thiol Detecting Reagent (in DMSO) 1 x 200µl -
Research areas
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Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. -
Pathway
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. -
Involvement in disease
Defects in AHCY are the cause of hypermethioninemia with S-adenosylhomocysteine hydrolase deficiency (HMAHCHD) [MIM:613752]. A metabolic disorder characterized by hypermethioninemia associated with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy. -
Sequence similarities
Belongs to the adenosylhomocysteinase family. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Alternative names
- Adenosylhomocysteinase
- AdoHcyase
- ahcY
see all
Images
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Adenosylhomocysteinase (AHCY) Inhibitor Screening Kit (Fluorometric) (ab204694)
Inhibition of Adenosylhomocysteinase (AHCY) Enzyme activity by 3-Deazaneplanocin A. IC50 of 3-Deazaneplanocin A was determined to be 0.137 nM.
