Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP139] to PI 3 Kinase catalytic subunit alpha/PIK3CA - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free
    See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies

  • Description

    Rabbit monoclonal [SP139] to PI 3 Kinase catalytic subunit alpha/PIK3CA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Chicken, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC: Human breast carcinoma tissue Flow Cyt: HeLa, and C6 cells. ICC/IF: HeLa, and C6 cells.

  • General notes

    ab243914 is the carrier-free version of ab135384.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:25(10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225716).
  • Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Flow Cytometry analysis of C6(Rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225716).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab135384 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab135384).

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:25(10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135384).
  • Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab135384).
  • Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)
    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] - BSA and Azide free (ab243914)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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