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Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP139] to PI 3 Kinase catalytic subunit alpha/PIK3CA
  • Suitable for: ICC, IHC-P, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139]
    See all PI 3 Kinase catalytic subunit alpha/PIK3CA primary antibodies

  • Description

    Rabbit monoclonal [SP139] to PI 3 Kinase catalytic subunit alpha/PIK3CA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    Human
    ICC
    Rat
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PI 3 Kinase catalytic subunit alpha/PIK3CA aa 500-600 (internal sequence). The exact sequence is proprietary.
    Database link: P42336

  • Positive control

    • IHC: Human breast carcinoma Tissue Flow Cyt: HeLa, and C6 cells. ICC: HeLa, and C6 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Immunocytochemistry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:25(10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Flow Cytometry analysis of C6(Rat glial tumor glial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labelling PI 3 Kinase catalytic subunit alpha/PIK3CA with ab135384 at 1/100 dilution.

  • Immunocytochemistry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Immunocytochemistry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1/25 dilution (10 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. Confocal image showing cytoplasmic staining in HeLa cells.

  • Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Flow Cytometry - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PI 3 Kinase catalytic subunit alpha/PIK3CA with purified ab135384 at 1:250 dilution (1.00 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  • Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)
    Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [SP139] (ab135384)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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