Anti-AMPK beta 1 (phospho S108) antibody (ab156890)
Key features and details
- Rabbit polyclonal to AMPK beta 1 (phospho S108)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AMPK beta 1 (phospho S108) antibody
See all AMPK beta 1 primary antibodies -
Description
Rabbit polyclonal to AMPK beta 1 (phospho S108) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in HEK293 Oligomycin 1µM whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species Flow Cyt HumanAll applications SheepHorseCowPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB 1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).ELISA Use a concentration of 1 µg/ml.Notes WB
1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 30 kDa).ELISA
Use a concentration of 1 µg/ml.Target
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Function
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). -
Sequence similarities
Belongs to the 5'-AMP-activated protein kinase beta subunit family. -
Domain
The glycogen-binding domain may target AMPK to glycogen so that other factors like glycogen-bound debranching enzyme or protein phosphatases can directly affect AMPK activity. -
Post-translational
modificationsPhosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. - Information by UniProt
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Database links
- Entrez Gene: 534107 Cow
- Entrez Gene: 5564 Human
- Entrez Gene: 100173201 Orangutan
- Omim: 602740 Human
- SwissProt: Q5BIS9 Cow
- SwissProt: Q9Y478 Human
- SwissProt: Q5R801 Orangutan
- Unigene: 726001 Human
see all -
Alternative names
- 1300015D22Rik antibody
- 5''-AMP-activated protein kinase subunit beta-1 antibody
- 5'-AMP-activated protein kinase beta-1 subunit antibody
see all
Images
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Western blot - Anti-AMPK beta 1 (phospho S108) antibody (ab156890)All lanes : Anti-AMPK beta 1 (phospho S108) antibody (ab156890) at 1/250 dilution
Lane 1 : Human HEK293 Oligomycin 1µM
Lane 2 : Human HEK293 Oligomycin 1µM with Immunising peptide at 1/250 dilution
Lane 3 : Human HEK293 Oligomycin 1µM with Control peptide at 1/250 dilution
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThe predicted molecular weight of AMPK beta 1 is 30 kDa (SwissProt), however we expect to observe a banding pattern at 38 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156890 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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ELISA - Anti-AMPK beta 1 (phospho S108) antibody (ab156890)ab156890 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
Datasheets and documents
References (0)
ab156890 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-AMPK beta 1 (phospho S108) antibody (ab156890)All lanes : Anti-AMPK beta 1 (phospho S108) antibody (ab156890) at 1/250 dilution
Lane 1 : Human HEK293 Oligomycin 1µM
Lane 2 : Human HEK293 Oligomycin 1µM with Immunising peptide at 1/250 dilution
Lane 3 : Human HEK293 Oligomycin 1µM with Control peptide at 1/250 dilution
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThe predicted molecular weight of AMPK beta 1 is 30 kDa (SwissProt), however we expect to observe a banding pattern at 38 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156890 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
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ELISA - Anti-AMPK beta 1 (phospho S108) antibody (ab156890)
ab156890 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
