Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)
![Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)](https://www.abcam.com/ps/products/68/ab68206/Images/ab68206-82402-anti-ampk-beta-1-phospho-s181-antibody-ep2181y-western-blot.jpg "Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)")
Key features and details
- Rabbit monoclonal [EP2181Y] to AMPK beta 1 (phospho S181)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y]
See all AMPK beta 1 primary antibodies -
Description
Rabbit monoclonal [EP2181Y] to AMPK beta 1 (phospho S181) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain tissue lysate (cells treated with AP). IHC-P: Human skeletal muscle. ICC: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2181Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)All lanes : Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206) at 1/20000 dilution
Lane 1 : human brain tissue lysate (cells were untreated)
Lane 2 : human brain tissue lysate (cells were treated with AP)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa
Observed band size: 32 and 45 kDa why is the actual band size different from the predicted?
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![Immunocytochemistry/ Immunofluorescence - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)](https://www.abcam.com/ps/products/68/ab68206/Images/ab68206-82401-anti-ampk-beta-1-phospho-s181-antibody-ep2181y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)Immunofluorescent staining of HeLa cells using ab68206 at 1/100-1/250 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)](https://www.abcam.com/ps/products/68/ab68206/Images/ab68206-82400-anti-ampk-beta-1-phospho-s181-antibody-ep2181y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK beta 1 (phospho S181) antibody [EP2181Y] (ab68206)Immunohistochemical analysis of paraffin-embedded human skeletal muscle using ab68206 at 1/100-1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
