Anti-PI4 kinase beta/PI4KB antibody (ab134756)
Key features and details
- Rabbit polyclonal to PI4 kinase beta/PI4KB
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PI4 kinase beta/PI4KB antibody
See all PI4 kinase beta/PI4KB primary antibodies -
Description
Rabbit polyclonal to PI4 kinase beta/PI4KB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human PI4 kinase beta/PI4KB aa 1-50 (N terminal). (CAA09495.1)
Sequence:MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK
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General notes
This product was previously labelled as PI4 kinase beta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0 to 8.0 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PI4 kinase beta/PI4KB antibody (ab134756)All lanes : Anti-PI4 kinase beta/PI4KB antibody (ab134756) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 10 seconds
Western Blot Gel 4-8% -
Immunoprecipitation - Anti-PI4 kinase beta/PI4KB antibody (ab134756)Lane 1: Detection of Human PI4 kinase beta/PI4KB by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134756 at 6 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized a downstream epitope of PI4 kinase beta/PI4KB.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134756 at 1 µg/ml.
Western Blot Gel 4-8%
Developed using the ECL technique with an exposure time of 3 seconds.
