Anti-VPS34 antibody [EPR5301] (ab124905)
![Anti-VPS34 antibody [EPR5301] (ab124905)](https://www.abcam.com/ps/products/124/ab124905/Images/ab124905-138886-anti-pi-3-kinase-class-3-antibody-epr5301-western-blot.jpg "Anti-VPS34 antibody [EPR5301] (ab124905)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5301] to VPS34
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-VPS34 antibody [EPR5301]
See all VPS34 primary antibodies -
Description
Rabbit monoclonal [EPR5301] to VPS34 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
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Immunogen
Synthetic peptide within Human VPS34 aa 800-900. The exact sequence is proprietary.
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Positive control
- L6, C2C12, 293T, SHYSY-5Y cell lysates; Mouse brain tissue lysate; Human gastric adenocarcinoma tissue
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General notes
This product was previously labelled as PI 3 Kinase Class 3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.27 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5301 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VPS34 antibody [EPR5301] (ab124905)All lanes : Anti-VPS34 antibody [EPR5301] (ab124905) at 1/1000 dilution
Lane 1 : L6 cell lysate
Lane 2 : C2C12 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : SHSY-5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 102 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS34 antibody [EPR5301] (ab124905)](https://www.abcam.com/ps/products/124/ab124905/Images/ab124905-138885-anti-pi-3-kinase-class-3-antibody-epr5301-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPS34 antibody [EPR5301] (ab124905)ab124905, at 1/50 dilution, staining PI 3 Kinase in paraffin-embedded Human gastric adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-VPS34 antibody [EPR5301] (ab124905)](https://www.abcam.com/ps/products/124/ab124905/Images/ab124905-138884-anti-pi-3-kinase-class-3-antibody-epr5301.jpg)
OI-RD Scanning - Anti-VPS34 antibody [EPR5301] (ab124905) -
![Anti-VPS34 antibody [EPR5301] (ab124905)](https://www.abcam.com/ps/products/124/ab124905/Images/ab124905-4-benefits-of-recombinant-antibodies.png)
Anti-VPS34 antibody [EPR5301] (ab124905)
