Anti-VPS34 antibody - N-terminal (ab227861)
Key features and details
- Rabbit polyclonal to VPS34 - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VPS34 antibody - N-terminal
See all VPS34 primary antibodies -
Description
Rabbit polyclonal to VPS34 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis
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Immunogen
Recombinant fragment within Human VPS34 (N terminal). The exact sequence is proprietary.
Database link: Q8NEB9 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell lysates; Wild-type and VPS34 transfected HEK-293T whole cell extracts. IP: HeLa whole cell extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VPS34 antibody - N-terminal (ab227861)All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : VPS34 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-VPS34 antibody - N-terminal (ab227861)All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-VPS34 antibody - N-terminal (ab227861)All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 whole cell lysate
Lane 4 : SK-N-AS whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-VPS34 antibody - N-terminal (ab227861)All lanes : Anti-VPS34 antibody - N-terminal (ab227861) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : VPS34 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 102 kDa5% SDS-PAGE gel.
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Immunoprecipitation - Anti-VPS34 antibody - N-terminal (ab227861)VPS34 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg of ab227861. Western blot was performed from the immunoprecipitate using ab227861. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab227861 IP in HeLa whole cell extract.
