Anti-Mucin 5AC antibody [CLH2] (ab77576)
![Anti-Mucin 5AC antibody [CLH2] (ab77576)](https://www.abcam.com/ps/products/77/ab77576/Images/picture 1.jpg "Anti-Mucin 5AC antibody [CLH2] (ab77576)")
Key features and details
- Mouse monoclonal [CLH2] to Mucin 5AC
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mucin 5AC antibody [CLH2]
See all Mucin 5AC primary antibodies -
Description
Mouse monoclonal [CLH2] to Mucin 5AC -
Host species
Mouse -
Specificity
ab77576 is specific for the MUC5AC tandem repeat of human mucin. No cross reactivity with other peptides from MUC2, 3, 6 or 7 The antibody recognises glycosylated as well as unglycosylated MUC5AC. -
Tested applications
Suitable for: IHC-Fr, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthetic peptide of the MUC5AC tandem repeat (Human).
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Positive control
- Native mucin extract from normal gastric mucosa and from gastric diffuse carcinoma.
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General notes
This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CLH2 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [CLH2] (ab77576)
ab77576 staining of foveolar epithelium of human gastric mucosa using IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex. Colour Development by DAB. Hematoxylin counterstained.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [CLH2] (ab77576)](https://www.abcam.com/ps/products/77/ab77576/Images/picture 2.jpg)
Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [CLH2] (ab77576)ab77576 staining of foveolar epithelium of human gastric mucosa by IHC-Fr. Detection with a biotin-conjugated rabbit anti-mouse secondary, followed by avidin-biotin peroxidase complex.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Mucin 5AC antibody [CLH2] (ab77576)](https://www.abcam.com/ps/products/77/ab77576/Images/Mucin-5AC-Primary-antibodies-ab77576-1.jpg)
Flow Cytometry - Anti-Mucin 5AC antibody [CLH2] (ab77576)Overlay histogram showing A549 cells stained with ab77576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77576, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This image was generated using the ascites version of the product.
