Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (ab273344)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Glyceraldehyde 3-Phosphate Assay Kit (Fluorometric) (ab273344) is a robust assay for the measurement of Glyceraldehyde 3-Phosphate (GA3P) in biological samples. The GA3P developer oxidizes GA3P with the release of NADH, which is used by the GA3P enzyme mix to convert the non-fluorescent GA3P probe to a fluorescent product measured at Ex/Em = 535/587 nm.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests GA3P Assay Buffer 1 x 25ml GA3P Developer Lyophilized 1 vial GA3P Enzyme Mix Lyophilized 1 vial GA3P Probe 1 x 400µl GA3P Standard Lyophilized 1 vial
Images
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Example data
Example data.
Kinetics of enzymatic reaction to measure GA3P in rat muscle (608 ng) and mouse kidney (8.5 µg) lysate with and without GA3P Standard spikes.
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Example dataExample data.
Interpolation of kinetic data for mouse kidney lysate. Δ GA3P = (GA3P spiked – GA3P unspiked).
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Example dataExample data.
GA3P in rat muscle and mouse kidney lysates.