Anti-Alanine Transaminase antibody (ab236658)
Key features and details
- Rabbit polyclonal to Alanine Transaminase
- Suitable for: IP, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Alanine Transaminase antibody
See all Alanine Transaminase primary antibodies -
Description
Rabbit polyclonal to Alanine Transaminase -
Host species
Rabbit -
Tested applications
Suitable for: IP, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Alanine Transaminase aa 1 to the C-terminus.
Database link: P24298 -
Positive control
- WB: Rat heart and liver tissue lysates; Mouse liver tissue lysates; HepG2 whole cell lysate. IHC-P: Human kidney and liver tissue. ICC/IF: HepG2 cells. IP: Mouse liver tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Alanine Transaminase antibody (ab236658) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Mouse liver tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
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Paraffin-embedded human liver tissue stained for Alanine Transaminase using ab236658 at 1/350 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Alanine Transaminase was immunoprecipitated from 500 μg of mouse liver tissue lysate using ab236658 at 1/200 dilution. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Lane 1: Rabbit control IgG instead of ab236658 in mouse liver lysate.
Lane 2: ab236658 IP in mouse liver lysate.
Lane 3: Mouse liver tissue lysate (input). -
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Alanine Transaminase (green) using ab236658 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human kidney tissue stained for Alanine Transaminase using ab236658 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-Alanine Transaminase antibody (ab236658) at 1/500 dilution
Lane 1 : Rat heart tissue lysate
Lane 2 : Rat liver tissue lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 55 kDa