Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p85 alpha (phospho Y607)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody
See all PI 3 Kinase p85 alpha primary antibodies -
Description
Rabbit polyclonal to PI 3 Kinase p85 alpha (phospho Y607) -
Host species
Rabbit -
Specificity
ab182651 detects endogenous level of PI 3 Kinase p85 only when phosphorylated at Tyrosine 607.
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PI 3 Kinase p85 alpha (phospho Y607) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Tyrosine 607 (D-Q-Y(p)-S-L) derived from Human PI3K p85.
Database link: P27986 -
Positive control
- WB: Insulin treated HeLa cell lysates. HeLa cells. Rat liver extract. MCF-7 cell lysate; mouse brain tissue lysate; rat brain tissue lysate. IHC-P: Human brain tissue.
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General notes
This product may cross-react with family member PIK3R2 due to the high homology with this protein around the phosphosite.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab182651 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/500 dilution
Lane 1 : Insulin treated HeLa cell lysates
Lane 2 : HeLa cell lysates
Secondary
All lanes : HRP-conjugate secondary antibody
Predicted band size: 84 kDaBlocking solution: 5% non-fat milk
Blocking for 1 hour at room temperature
Primary incubation overnight at 4°C
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)Immunohistochemistry of human brain tissue labeling PI 3 Kinase p85 (phospho Y607) with ab182651 at 1/200.
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Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/500 dilution
Lane 1 : Rat liver extract
Lane 2 : Rat liver extract with antigen-specific peptide
Predicted band size: 84 kDa
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Western blot - Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651)All lanes : Anti-PI 3 Kinase p85 alpha (phospho Y607) antibody (ab182651) at 1/750 dilution
Lane 1 : MCF-7 cell lysate with N-Peptide
Lane 2 : MCF-7 cell lysate
Lane 3 : MCF-7 cell lysate with P-Peptide
Lane 4 : Mouse brain tissue lysate with N-Peptide
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse brain tissue lysate with P-Peptide
Lane 7 : Rat brain tissue lysate with N-Peptide
Lane 8 : Rat brain tissue lysate
Lane 9 : Rat brain tissue lysate with P-Peptide
Lysates/proteins at 40 µg per lane.
Predicted band size: 84 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
