Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP62] to PI 3 Kinase p85 alpha - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free
    See all PI 3 Kinase p85 alpha primary antibodies

  • Description

    Rabbit monoclonal [SP62] to PI 3 Kinase p85 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow

  • Immunogen

    Synthetic peptide within Human PI 3 Kinase p85 alpha aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P27986

  • Positive control

    • IHC-P: Human placenta tissue ICC/IF: NIH/3T3 and MCF7 cells Flow Cyt: PC-12, NIH/3T3 and MCF7 cells

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab236440 is the carrier-free version of ab225720. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab236440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of MCF-7 (human breast carcinoma) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 dilution (10.32 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/2500 dilution (1.03 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 dilution (10.32 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Flow Cytometry - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cell line labeling PI 3 Kinase p85 alpha with ab225720 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human testis tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human breast adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225720).

  • Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)
    Anti-PI 3 Kinase p85 alpha antibody [SP62] - BSA and Azide free (ab236440)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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