Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
![Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)](https://www.abcam.com/ps/products/247/ab247290/Images/ab247290-7-anti-neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP673Y] to Neurofilament heavy polypeptide - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free
See all Neurofilament heavy polypeptide primary antibodies -
Description
Rabbit monoclonal [EP673Y] to Neurofilament heavy polypeptide - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody recognises Neuofilaments (NF).
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Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247290 is the carrier-free version of ab40796. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247290 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP673Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
This data was developed using ab40796, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain glyoma tissue labelling Neurofilament heavy polypeptide with unpurified ab40796. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)](https://www.abcam.com/ps/products/247/ab247290/Images/ab247290-410506-anti-neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (purified) + Human brain tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa why is the actual band size different from the predicted?This data was developed using ab40796, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)](https://www.abcam.com/ps/products/247/ab247290/Images/ab247290-407849-anti-neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)All lanes : Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 112 kDa
Observed band size: 200-220 kDa why is the actual band size different from the predicted?This data was developed using ab40796, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)](https://www.abcam.com/ps/products/247/ab247290/Images/ab247290-406322-anti-neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (unpurified) + Rat brain tissue lysate
Predicted band size: 112 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.This data was developed using ab40796, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)](https://www.abcam.com/ps/products/247/ab247290/Images/ab247290-1-anti-neurofilament-heavy-polypeptide-antibody-ep673y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)This data was developed using ab40796, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling Neurofilament heavy polypeptide with purified ab40796 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. -
![Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)](https://www.abcam.com/ps/products/247/ab247290/Images/ab247290-5-benefits-of-recombinant-antibodies.png)
Anti-Neurofilament heavy polypeptide antibody [EP673Y] - BSA and Azide free (ab247290)
