Recombinant E. coli Carbonic anhydrase 2/CA2 protein (ab208309)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies, MS
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Product name
Recombinant E. coli Carbonic anhydrase 2/CA2 protein
See all Carbonic anhydrase 2/CA2 proteins and peptides -
Biological activity
Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
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Purity
> 95 % SDS-PAGE.
ab208309 was purified by conventional chromatography techniques. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Escherichia coli -
Sequence
MGSSHHHHHHSSGLVPRGSHMKDIDTLISNNALWSKMLVEEDPGFFEKLA QAQKPRFLWIGCSDSRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSV VQYAVDVLEVEHIIICGHYGCGGVQAAVENPELGLINNWLLHIRDIWFKH SSLLGEMPQERRLDTLCELNVMEQVYNLGHSTIMQSAWKRGQKVTIHGWA YGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHANHK -
Predicted molecular weight
27 kDa including tags -
Amino acids
1 to 220 -
Tags
His tag N-Terminus -
Additional sequence information
NP_414668
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.02% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - Recombinant E. coli Carbonic anhydrase 2/CA2 protein (ab208309)
15% SDS-PAGE analysis of 3 µg ab208309.