Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free
See all Thioredoxin / TRX primary antibodies
Description

Rabbit monoclonal [EPR23843-48] to Thioredoxin / TRX - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Rat
ICC	Mouse
IHC-P	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NIH/3T3, Neuro 2a, C2C12 and PC-12 whole cell lysates; Mouse kidney, liver and testis tissue lysates; Rat kidney tissue lysate. IHC-P: Mouse testis and kidney tissue; Rat kidney tissue. ICC: NIH/3T3 and C6 cells. Flow cyt: NIH/3T3 and C6 cells.
General notes
ab273880 is the carrier-free version of ab270449. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23843-48
Isotype

IgG
Research areas

Immunocytochemistry - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Thioredoxin / TRX with ab273877 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Thioredoxin / TRX with abab273877 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat kidney. The section was incubated with ab273877 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Flow Cytometry - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling Thioredoxin / TRX with ab273877 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Anti-Thioredoxin / TRX antibody [EPR23843-48] (ab273877) at 1/1000 dilution + Rat kidney tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 12 kDa
Observed band size: 11 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

All lanes : Anti-Thioredoxin / TRX antibody [EPR23843-48] (ab273877) at 1/1000 dilution

Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Mouse testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 12 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin / TRX with abab273877 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID: 20682242). The section was incubated with ab273877 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Western blot - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

All lanes : Anti-Thioredoxin / TRX antibody [EPR23843-48] (ab273877) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 3 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 12 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Lane 1, 2: 7.75 seconds Lane 3, 4: 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Flow Cytometry - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Thioredoxin / TRX with ab273877 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Immunocytochemistry - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C6 cells labelling Thioredoxin / TRX with ab273877 at 1/250 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in C6 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2 ug/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Thioredoxin / TRX with abab273877 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on sertoli and leydig cells of mouse testis. The section was incubated with ab273877 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273877).
Anti-Thioredoxin / TRX antibody [EPR23843-48] - BSA and Azide free (ab273880)