Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TRK fused gene antibody [EPR8766] (ab156866)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8766] to TRK fused gene
Suitable for: WB, ICC/IF, IHC-P, IP
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-TRK fused gene antibody [EPR8766]
See all TRK fused gene primary antibodies
Description

Rabbit monoclonal [EPR8766] to TRK fused gene
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human TRK fused gene. The exact sequence is proprietary.
Positive control
- WB: Human fetal brain tissue lysate and HeLa, A549, 293T, and wild-type HAP1 cell lysates. IHC-P: Human colon and thyroid carcinoma tissues. ICC/IF: HeLa and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8766
Isotype

IgG
Research areas

Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)

All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TFG knockout HeLa cell lysate
Lane 3 : HAP-1 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 43 kDa
Observed band size: 57 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa.

ab156866 Anti-TRK fused gene antibody [EPR8766] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used. Wild-type and TFG knockout samples were subjected to SDS-PAGE. ab156866 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)

All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TFG knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 43 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab156866 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab156866 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween^®) before incubation with ab156866 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-TRK fused gene antibody [EPR8766] (ab156866)

All lanes : Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling TRK fused gene with purified ab156866 at 1/1000. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue).

Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Immunoprecipitation - Anti-TRK fused gene antibody [EPR8766] (ab156866)

Immunoprecipitaion analysis in 293T cell lysate of TRK fused gene immunoprecipitated with ab156866 at 1/10.

Anti-TRK fused gene antibody [EPR8766] (ab156866) at 1/10000 dilution + Immunoprecipitation pellets from 293T cell lysate at 10 µg
Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8766] (ab156866)

Immunofluorescent analysis of HeLa cells labeling TRF fused gene with ab156866 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TRF fused gene with ab156966 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8766] (ab156866)

Immunohistochemical analysis of paraffin embedded Human thyroid carcinoma tissue labeling TRF fused gene with ab156866 at 1/50.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
OI-RD Scanning - Anti-TRK fused gene antibody [EPR8766] (ab156866)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-TRK fused gene antibody [EPR8766] (ab156866)

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