Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TRK fused gene antibody [EPR8765(2)(B)]
    See all TRK fused gene primary antibodies

  • Description

    Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TRK fused gene aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: Human fetal brain tissue lysate, wild-type HAP1, 293T and HeLa cell lysates. IHC-P: Human colon tissue. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : TFG knockout HeLa cell lysate
    Lane 3 : HAP-1 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used.  Wild-type and TFG knockout samples were subjected to SDS-PAGE.  ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TFG knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab150428 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TRK fused gene with ab150428 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunocytochemistry/ Immunofluorescence - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunofluorescent analysis of HeLa cells labelling TRK fused gene with ab150428 at 1/250 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

    Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab150428 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

    Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150428 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

    Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150428 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

    Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150428 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150428 showing +ve staining.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 43 kDa

  • Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)
    Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428)

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