Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free
    See all TRK fused gene primary antibodies

  • Description

    Rabbit monoclonal [EPR8765(2)(B)] to TRK fused gene - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain tissue lysate, wild-type HAP1, MCF7, HEK293T and HeLa cell lysates. IHC-P: Human colon tissue. ICC/IF: HeLa cells.

  • General notes

    ab235872 is the carrier-free version of ab150428. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab235872 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
    Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
    All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : TFG knockout HeLa cell lysate
    Lane 3 : HAP-1 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 43 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab150428).

    Lanes 1-4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab150428 Anti-TRK fused gene antibody [EPR8765(2)(B)] was shown to specifically react with TFG in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265841 (knockout cell lysate ab257738) was used.  Wild-type and TFG knockout samples were subjected to SDS-PAGE.  ab150428 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
    Western blot - Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
    All lanes : Anti-TRK fused gene antibody [EPR8765(2)(B)] (ab150428) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : TFG knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab150428).

    Lanes 1 - 4: Merged signal (red and green). Green - ab150428 observed at 57 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab150428 was shown to react with TFG in HAP1 wild-type cells in Western blot. Loss of signal was observed when TFG knockout sample was used. HAP1 wild-type and TFG knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab150428 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)
    Anti-TRK fused gene antibody [EPR8765(2)(B)] - BSA and Azide free (ab235872)

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