Rat KIM-1 ELISA Kit (ab223858)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.33 pg/ml
- Range: 11.72 pg/ml - 750 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat KIM-1 ELISA Kit
See all TIM 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Urine 9 2.4% Inter-assay Sample n Mean SD CV% Urine 3 4.3% -
Sample type
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.33 pg/ml -
Range
11.72 pg/ml - 750 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 104 102% - 107% Serum 99 95% - 101% Cell culture media 102 100% - 105% Hep Plasma 102 98% - 105% EDTA Plasma 101 98% - 104% Cit plasma 100 98% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat KIM-1 ELISA Kit (ab223858) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of KIM-1 protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat KIM-1 with 2.33 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
KIM-1, also known as Kidney Injury Molecule 1 and Hepatitis A virus cellular receptor 1 homolog (HAVcr-1), or T-cell immunoglobulin and mucin domain 1 (TIM-1) is a 283- amino acid protein that contains an N-terminal immunoglobulin-like domain and is encoded by the HAVCR1 gene. KIM-1 is a single-pass type 1 membrane protein has been identified as a receptor for hepatitis A virus. The TIM gene family participates in host immune response. Urinary KIM-1 levels are elevated in nephropathy and is thought to be a biomarker for renal damage.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit 10X Rat KIM-1 Capture Antibody 1 x 600µl 10X Rat KIM-1 Detector Antibody 1 x 600µl Rat KIM-1 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
Tissue specificity
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
Sequence similarities
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD365
- HAVCR
- HAVCR 1
see all -
Database links
- Entrez Gene: 286934 Rat
- SwissProt: O54947 Rat
Images
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Other - Rat KIM-1 ELISA Kit (ab223858)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Rat KIM-1 standard curve comparison dataStandard curve comparison between rat KIM-1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of Rat KIM-1 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native KIM-1 in Rat serum, plasma, and urine samplesThe concentrations of KIM-1 were measured in duplicates, interpolated from the KIM-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 12.5%, and urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean KIM-1 concentration was determined to be 1,435 pg/mL in serum, 1,332 pg/mL in plasma (citrate), 1,444 pg/mL in plasma (EDTA), 1,726 pg/mL in plasma (heparin), and 2,005 pg/mL in urine.
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Interpolated concentrations of spiked KIM-1 in cell culture supernatant samplesThe concentrations of KIM-1 were measured in duplicate and interpolated from the KIM-1 standard curve and corrected for sample dilution. Undiluted samples are as follows: RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Rat KIM-1 ELISA Kit (ab223858)To learn more about the advantages of recombinant antibodies see here.
