Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23611-101] to cGAS - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-cGAS antibody [EPR23611-101] - BSA and Azide free
    See all cGAS primary antibodies

  • Description

    Rabbit monoclonal [EPR23611-101] to cGAS - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 and C2C12 IP: NIH/3T3 whole cell lysate.

  • General notes

    ab277486 is the carrier-free version of ab252416. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23611-101

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)
    Western blot - Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)
    All lanes : Anti-cGAS antibody [EPR23611-101] (ab252416) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : C2C12 (mouse myoblasts myoblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 58 kDa
    Observed band size: 58 kDa



    This data was developed using ab252416, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure times: Lane 1: 26 seconds Lane 2: 15 seconds.

     

  • Immunoprecipitation - Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)
    Immunoprecipitation - Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)

    This data was developed using ab252416, the same antibody clone in a different buffer formulation.

    cGAS was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug with ab252416 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252416 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug

    Lane 2: ab252416 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252416 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

  • Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)
    Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)

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