Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)
![Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)](https://www.abcam.com/ps/products/277/ab277486/Images/ab277486-415158-anti-cgas-antibody-epr23611101-western-blot-fibroblast.jpg "Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23611-101] to cGAS - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-cGAS antibody [EPR23611-101] - BSA and Azide free
See all cGAS primary antibodies -
Description
Rabbit monoclonal [EPR23611-101] to cGAS - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 and C2C12 IP: NIH/3T3 whole cell lysate.
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General notes
ab277486 is the carrier-free version of ab252416. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab277486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23611-101 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)All lanes : Anti-cGAS antibody [EPR23611-101] (ab252416) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDaThis data was developed using ab252416, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure times: Lane 1: 26 seconds Lane 2: 15 seconds.
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![Immunoprecipitation - Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)](https://www.abcam.com/ps/products/277/ab277486/Images/ab277486-2-anti-cgas-antibody-epr23611101-immunoprecipitation-nih3t3.jpg)
Immunoprecipitation - Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)This data was developed using ab252416, the same antibody clone in a different buffer formulation.
cGAS was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug with ab252416 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252416 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 10 ug
Lane 2: ab252416 IP in NIH/3T3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252416 in NIH/3T3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
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![Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)](https://www.abcam.com/ps/products/277/ab277486/Images/ab277486-447360-benefitsofrecombinantantibodies.png)
Anti-cGAS antibody [EPR23611-101] - BSA and Azide free (ab277486)
