Anti-PI 3 Kinase p85 alpha antibody (ab227204)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p85 alpha
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PI 3 Kinase p85 alpha antibody
See all PI 3 Kinase p85 alpha primary antibodies -
Description
Rabbit polyclonal to PI 3 Kinase p85 alpha -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human PI 3 Kinase p85 alpha (internal sequence). The exact sequence is proprietary.
Database link: P27986 -
Positive control
- WB: PC-12, Rat2, Neuro-2a, C8D30, NIH/3T3, Raw 264.7, C2C12, Jurkat, K562 and THP-1 whole cell extracts. IP: Jurkat whole cell extract. IHC-P: Mouse duodenum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204)All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell extract
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 83 kDa7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody (ab227204)Paraffin-embedded mouse duodenum stained for PI 3 Kinase p85 alpha with ab227204 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-PI 3 Kinase p85 alpha antibody (ab227204)PI 3 Kinase p85 alpha was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 µg ab227204. Western blot was performed from the immunoprecipitate using ab227204 at 1/500 dilution.
Lane 1: Control IgG IP in Jurkat whole cell extract.
Lane 2: ab227204 IP in Jurkat whole cell extract.
10% SDS-PAGE.
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Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204)All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 4 : Raw 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 83 kDa7.5% SDS-PAGE
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Western blot - Anti-PI 3 Kinase p85 alpha antibody (ab227204)All lanes : Anti-PI 3 Kinase p85 alpha antibody (ab227204) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 83 kDa7.5% SDS-PAGE
