Anti-CHMP1a antibody [EPR11939] (ab178686)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11939] to CHMP1a
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-CHMP1a antibody [EPR11939]
See all CHMP1a primary antibodies -
Description
Rabbit monoclonal [EPR11939] to CHMP1a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIP HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human CHMP1a aa 100 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9HD42 -
Positive control
- HeLa, 293T and MCF-7 cell lysates. Permeabilized 293T cells. Immunoprecipitation pellet from MCF-7 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11939 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CHMP1a antibody [EPR11939] (ab178686) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 22 kDa
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Flow cytometric analysis of permeabilized 293T cells using ab178686 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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Western blot analysis on immunoprecipitation pellet from (Lane 1) MCF-7 cell lysate or (Lane 2) 1XPBS (negative control) using ab178686 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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