Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11159] to Nogo Receptor - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free
    See all Nogo Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR11159] to Nogo Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SH-SY5Y, 293 and HepG2 cell lysates. Neuro-2a and C6 whole cell lysates. Mouse brain and hippocampus tissue lysates.

  • General notes

    Ab250758 is the carrier-free version of ab184556. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250758 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    Western blot - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/5000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse hippocampus tissue lysate
    Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa

  • Western blot - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    Western blot - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/50000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab184556, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    Western blot - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/10000 dilution + 293 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 66 kDa why is the actual band size different from the predicted?



    This data was developed using ab184556, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    Immunoprecipitation - Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)

    This data was developed using ab184556, the same antibody clone in a different buffer formulation.

    Western blot analysis of Nogo Receptor in HepG2 cell lysate immunoprecipitated with ab184556 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)
    Anti-Nogo Receptor antibody [EPR11159] - BSA and Azide free (ab250758)

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