Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)
![Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)](https://www.abcam.com/ps/products/239/ab239961/Images/ab239961-406078-anti-adipose-triglyceride-lipase-antibody-epr3444-2-bsa-and-azide-free-western-blot.jpg "Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3444(2)] to Adipose Triglyceride Lipase - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free
See all Adipose Triglyceride Lipase primary antibodies -
Description
Rabbit monoclonal [EPR3444(2)] to Adipose Triglyceride Lipase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab239961 is the carrier-free version of ab109251. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab239961 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3444(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/5000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab109251, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)](https://www.abcam.com/ps/products/239/ab239961/Images/ab239961-403248-anti-adipose-triglyceride-lipase-antibody-epr3444-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (purified)
Lane 1 : Human heart tissue lysate
Lane 2 : A431 whole cell lysate
Lane 3 : A673 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab109251, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)](https://www.abcam.com/ps/products/239/ab239961/Images/ab239961-398401-anti-adipose-triglyceride-lipase-antibody-epr3444-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (unpurified)
Lane 1 : 3T3-L1 lysate
Lane 2 : A431 lysate
Lane 3 : A673 lysate
Lane 4 : Human heart tissue lysate
Lane 5 : Human skeletal muscle tissue lysate
Lane 6 : Human adipose tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab109251, the same antibody clone in a different buffer formulation.
-
![Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)](https://www.abcam.com/ps/products/239/ab239961/Images/ab239961-4-benefits-of-recombinant-antibodies.png)
Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] - BSA and Azide free (ab239961)
