Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)
![Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)](https://www.abcam.com/ps/products/109/ab109251/Images/ab109251-253621-anti-adipose-triglyceride-lipase-antibody-epr34442-western-blot.jpg "Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3444(2)] to Adipose Triglyceride Lipase
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)]
See all Adipose Triglyceride Lipase primary antibodies -
Description
Rabbit monoclonal [EPR3444(2)] to Adipose Triglyceride Lipase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: 3T3-L1, A431 and A673 cell lysates, human heart, skeletal muscle and adipose tissue lysates. Mouse and rat heart tissue lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3444(2) -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/5000 dilution (purified)
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)](https://www.abcam.com/ps/products/109/ab109251/Images/ab109251-253620-anti-adipose-triglyceride-lipase-antibody-epr34442-western-blot.jpg)
Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (purified)
Lane 1 : Human heart tissue lysate
Lane 2 : A431 whole cell lysate
Lane 3 : A673 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
-
![Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)](https://www.abcam.com/ps/products/109/ab109251/Images/ab109251-121951-anti-adipose-triglyceride-lipase-antibody-epr34442-western-blot.jpg)
Western blot - Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)All lanes : Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251) at 1/1000 dilution (unpurified)
Lane 1 : 3T3-L1 lysate
Lane 2 : A431 lysate
Lane 3 : A673 lysate
Lane 4 : Human heart tissue lysate
Lane 5 : Human skeletal muscle tissue lysate
Lane 6 : Human adipose tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
-
![Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)](https://www.abcam.com/ps/products/109/ab109251/Images/ab109251-4-benefits-of-recombinant-antibodies.png)
Anti-Adipose Triglyceride Lipase antibody [EPR3444(2)] (ab109251)
