Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 09, 2021

Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18388-138] to p27 KIP 1
Suitable for: ICC/IF, WB, IHC-P, IP
Reacts with: Mouse, Rat

Product name

Anti-p27 KIP 1 antibody [EPR18388-138]
See all p27 KIP 1 primary antibodies
Description

Rabbit monoclonal [EPR18388-138] to p27 KIP 1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Mouse
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat thymus tissue lysate; NIH/3T3, C2C12, Neuro-2a and C6 whole cell lysate. ICC/IF: Neuro-2a and NIH/3T3 cells. IHC-P: Mouse kidney, mouse testis, rat testis and rat cerebral cortex tissue. IP: C2C12 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18388-138
Isotype

IgG
Research areas

Western blot - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

All lanes : Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851) at 1/1000 dilution

Lane 1 : Rat thymus tissue lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 4 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 5 : C6 (rat glial tumor cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 22 kDa
Observed band size: 18, 27 kDa
why is the actual band size different from the predicted?

Blocking/Dilution: 5% NFDM/TBST.

Exposure: Lanes 1 and 3: 30 seconds; Lanes 2 and 5: 10 seconds; Lane 4: 15 seconds.

The molecular mass observed is consistent with the literature (PMID: 9033255; PMID: 9679152). The lower band observed in some samples could be a degradation product (PMID: 9190912; 24229711).
Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma cell line) cells labeling p27 KIP 1 with ab190851 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly nuclear staining in the Neuro-2a cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling p27 KIP 1 with ab190851 at 1/40000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Nuclear staining on Leydig cells and Sertoli cells of mouse testis is observed (PMID: 10098522). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

p27 KIP 1 was immunoprecipitated from 0.35 mg of C2C12 (mouse myoblast cell line) whole cell lysate with ab190851 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190851 at 1/1000 dilution. VeriBlot for IP Detection Reagents (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: C2C12 whole cell lysate 10 µg (Input).

Lane 2: ab190851 IP in C2C12 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190851 in C2C12 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling p27 KIP 1 with ab190851 at 1/40000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Nuclear staining on mouse kidney is observed (PMID: 26823281). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling p27 KIP 1 with ab190851 at 1/40000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Nuclear staining on Sertoli cells and Leydig cells of rat testis is observed (PMID: 10098522). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling p27 KIP 1 with ab190851 at 1/40000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Nuclear staining in the rat cerebral cortex is observed (PMID: 19852587). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling p27 KIP 1 with ab190851 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly nuclear staining in the NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Anti-p27 KIP 1 antibody [EPR18388-138] (ab190851)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com