Anti-p27 KIP 1 antibody, prediluted (ab15491)
Key features and details
- Rabbit polyclonal to p27 KIP 1, prediluted
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p27 KIP 1 antibody, prediluted
See all p27 KIP 1 primary antibodies -
Description
Rabbit polyclonal to p27 KIP 1, prediluted -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
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Immunogen
Synthetic peptide within Human p27 KIP 1 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P46527 -
Positive control
- MAD109, Prostate and Colon. IF/ICC: A431 cell line
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-p27 KIP 1 antibody, prediluted (ab15491)
ICC/IF image of ab15491 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15491, Neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody, prediluted (ab15491)ab15491 staining p27 KIP 1 in human prostate by Immunohistochemistry (FFPE-sections).
