Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)
![Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)](https://www.abcam.com/ps/products/92/ab92741/Images/ab92741-107959-anti-p27-kip-1-antibody-epfhcr16-western-blot.jpg "Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPFHCR16] to p27 KIP 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-p27 KIP 1 antibody [EPFHCR16]
See all p27 KIP 1 primary antibodies -
Description
Rabbit monoclonal [EPFHCR16] to p27 KIP 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatWB Mouse
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Immunogen
Recombinant fragment corresponding to Mouse p27 KIP 1.
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Positive control
- WB: NIH3T3, C6 and Neuro 2a cell lysates. IHC-P: Rat testis, mouse testis, lung and thymus tissues.
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General notes
Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPFHCR16 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)All lanes : Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) at 1/1000 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Neuro 2a
cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)](https://www.abcam.com/ps/products/92/ab92741/Images/ab92741-293219-anti-p27-kip-1-antibody-epfhcr16-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of mouse testis is observed (PMID: 10098522). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody.
Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)](https://www.abcam.com/ps/products/92/ab92741/Images/ab92741-293220-anti-p27-kip-1-antibody-epfhcr16-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of rat testis is observed (PMID: 10098522). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody.
Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)](https://www.abcam.com/ps/products/92/ab92741/Images/ab92741-107958-anti-p27-kip-1-antibody-epfhcr16-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)ab92741 at 1/100 dilution staining p27 KIP in Mouse lung by Immunohistochemistry, Paraffin-embedded tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)](https://www.abcam.com/ps/products/92/ab92741/Images/ab92741-107957-anti-p27-kip-1-antibody-epfhcr16-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)ab92741 at 1/100 dilution staining p27 KIP in Mouse thymus by Immunohistochemistry, Paraffin-embedded tissue.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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![Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)](https://www.abcam.com/ps/products/92/ab92741/Images/ab92741-6-benefits-of-recombinant-antibodies.png)
Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)
