Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Thursday March 04, 2021

Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPFHCR16] to p27 KIP 1
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat

Product name

Anti-p27 KIP 1 antibody [EPFHCR16]
See all p27 KIP 1 primary antibodies
Description

Rabbit monoclonal [EPFHCR16] to p27 KIP 1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

Rat

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment corresponding to Mouse p27 KIP 1.
Positive control
- WB: NIH3T3, C6 and Neuro 2a cell lysates. IHC-P: Rat testis, mouse testis, lung and thymus tissues.
General notes
Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Mouse Rat
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPFHCR16
Isotype

IgG
Research areas

Western blot - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

All lanes : Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741) at 1/1000 dilution

Lane 1 : NIH3T3 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Neuro 2a
cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 22 kDa
Observed band size: 27 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of mouse testis is observed (PMID: 10098522). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody.

Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling p27 KIP 1 with ab92741 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in Leydig and Sertoli cells and Leydig cells of rat testis is observed (PMID: 10098522). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody.

Heat mediated antigen retrieval was performed using Universal HIER antigen retrieval reagent (10X) (ab208572).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

ab92741 at 1/100 dilution staining p27 KIP in Mouse lung by Immunohistochemistry, Paraffin-embedded tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

ab92741 at 1/100 dilution staining p27 KIP in Mouse thymus by Immunohistochemistry, Paraffin-embedded tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-p27 KIP 1 antibody [EPFHCR16] (ab92741)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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