Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
See all PHD1/prolyl hydroxylase primary antibodies
Description

Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- A549,HeLa, and MCF-7 cell lysates Human ovarian adenocarcinoma tissue Human testis tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2745
Isotype

IgG
Research areas

Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)

All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilution

Lane 1 : A549 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)

Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)

Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)

Immunofluorescent staining of HeLa cells using ab108980
Flow Cytometry - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)

Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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