Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
See all PHD1/prolyl hydroxylase primary antibodies -
Description
Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549,HeLa, and MCF-7 cell lysates Human ovarian adenocarcinoma tissue Human testis tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2745 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilution
Lane 1 : A549 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa
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Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab108980
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Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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