Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2746] to PHD1/prolyl hydroxylase
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-PHD1/prolyl hydroxylase antibody [EPR2746]
See all PHD1/prolyl hydroxylase primary antibodies
Description

Rabbit monoclonal [EPR2746] to PHD1/prolyl hydroxylase
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A549, HeLa and MCF-7 cell lysates, Human fetal heart, Mouse brain, Mouse heart, Rat brain, Rat heart tissue lysates IHC-P: Mouse testis, Rat spleen, Human breast carcinoma and Human lung carcinoma tissue ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2746
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1:50 dilution (5.14 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PHD1/prolyl hydroxylase with purified ab113077 at 1/50 dilution (5 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1:50 dilution (5.14 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1:50 dilution (5.14 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1:30 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077) at 1/1000 dilution (Purified)

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 44 kDa
Observed band size: 40,43 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077) at 1/1000 dilution

Lane 1 : A549 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 44 kDa

This image was generated using the unpurified version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.

This image was generated using the unpurified version of the product.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)

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