Anti-ALK-1 antibody [EPR22985-108] (ab263902)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22985-108] to ALK-1
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-ALK-1 antibody [EPR22985-108]
See all ALK-1 primary antibodies -
Description
Rabbit monoclonal [EPR22985-108] to ALK-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant Mouse ALK-1 Fc Chimera protein; Mouse lung and placenta lysates; rat lung lysates; bEnd.3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22985-108 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ALK-1 antibody [EPR22985-108] (ab263902) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse placenta lysate
Lane 3 : Rat lung lysate
Lane 4 : Rat placenta lysate
Lane 5 : bEnd.3 (mouse brain endothelioma) whole cell lysate
Lane 6 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 56,63 kDa why is the actual band size different from the predicted?Blockinmg and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
There are two isoforms. Different isoforms show different content in different tissues.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11927558, 7488127).Negative control: MEF (PMID: 11927558).
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Anti-ALK-1 antibody [EPR22985-108] (ab263902) at 1/1000 dilution + Recombinant Mouse ALK-1 Fc Chimera protein (aa23-119), 20 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 8 seconds.
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