Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13064(B)] to TRP1 - C-terminal
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-TRP1 antibody [EPR13064(B)] - C-terminal
See all TRP1 primary antibodies -
Description
Rabbit monoclonal [EPR13064(B)] to TRP1 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
Synthetic peptide within Human TRP1 (C terminal) (Cysteine residue). The exact sequence is proprietary.
Database link: P17643 -
Positive control
- Human melanoma and Human skin lysates. Human melanoma tissue and Human skin tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13064(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-TRP1 antibody [EPR13064(B)] - C-terminal (ab177468) at 1/1000 dilution
Lane 1 : Human melanoma lysate
Lane 2 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
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Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling TRP1 with ab177468 at 1/50 diultion.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from fetal skin lysate using ab177468 at 1/10 dilution.
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Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab177468 at 1/50 diultion.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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