Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TRP1 antibody [EPR21960] (ab235447)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21960] to TRP1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-TRP1 antibody [EPR21960]
See all TRP1 primary antibodies
Description

Rabbit monoclonal [EPR21960] to TRP1
Host species

Rabbit
Specificity

We recommend WB for human and mouse; we predict it will also work in WB with rat lysates but were unable to test this.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
ICC/IF	Mouse Human
IHC-P	Mouse Rat Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human skin and melanoma tissues; Mouse and rat skin tissues. WB: MeWo and B16-F0 whole cell lysates. ICC/IF: MeWo and B16-F0 cells. Flow Cyt: MeWo and B16-F0 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21960
Isotype

IgG
Research areas

Western blot - Anti-TRP1 antibody [EPR21960] (ab235447)

All lanes : Anti-TRP1 antibody [EPR21960] (ab235447) at 1/1000 dilution

Lane 1 : MeWo (human malignant melanoma fibroblast cell ine) whole cell lysate
Lane 2 : B16-F0 (mouse melanoma epithelial cell-like cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Exposure time: Lane 1: 10 seconds; Lane 2: 3 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The observed mass is greater than the predicted due to glycosylation.

The molecular mass observed is consistent with what has been described in the literature (PMID: 21324755; PMID: 18042623;18650849).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of melanocytes in human skin (PMID:21562572). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunofluorescent analysis of 100% methanol fixed B16-F0 (mouse melanoma epithelial cell-like cell line) cells labeling TRP1 with ab235447 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in the B16-F0 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-TRP1 antibody [EPR21960] (ab235447)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized B16-F0 (mouse melanoma epithelial cell-like cell line) cells labeling TRP1 with ab235447 at 1/60 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of melanocytes in rat skin (PMID: 21562572). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of melanocytes in mouse skin (PMID: 21562572). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP1 with ab235447 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in tumor cells of human melanoma (PMID: 22045183; 26068396). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-TRP1 antibody [EPR21960] (ab235447)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MeWo (human malignant melanoma fibroblast cell line) cells labeling TRP1 with ab235447 at 1/60 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

TRP1 is predominantly expressed in mature melanosomes (PMID: 27073483, 11266471).
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR21960] (ab235447)

Immunofluorescent analysis of 100% methanol fixed MeWo (human malignant melanoma fibroblast cell line) cells labeling TRP1 with ab235447 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in MeWo cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-TRP1 antibody [EPR21960] (ab235447)

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