Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 09, 2021

Anti-TRP1 antibody [EPR21956] (ab235446)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21956] to TRP1
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-TRP1 antibody [EPR21956]
See all TRP1 primary antibodies
Description

Rabbit monoclonal [EPR21956] to TRP1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MeWo, SK-MEL-2 and SK-MEL-28 whole cell lysates. IHC-P: Human skin and melanoma tissue. ICC/IF: MeWo cells. Flow cyt: MeWo cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21956
Isotype

IgG
Research areas

Western blot - Anti-TRP1 antibody [EPR21956] (ab235446)

All lanes : Anti-TRP1 antibody [EPR21956] (ab235446) at 1/1000 dilution

Lane 1 : MeWo (human malignant melanoma cell line) whole cell lysate
Lane 2 : SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate
Lane 3 : SK-MEL-28 (human malignant melanoma cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 61 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 8 seconds, Lanes 2 & 3: 3 minutes.

The observed mass is greater than the predicted due to glycosylation.
The molecular mass observed is consistent with what has been described in the literature (PMID: 21324755; PMID: 18042623; 18650849).
The blot with lanes 2 and 3 was developed with a high sensitivity ECL substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21956] (ab235446)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP1 with ab235446 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of melanocytes in human skin (PMID:21562572) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21956] (ab235446)

Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP1 with ab235446 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in tumor cells of human melanoma (PMID: 22045183; 26068396) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-TRP1 antibody [EPR21956] (ab235446)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MeWo (human malignant melanoma cell line) cells labeling TRP1 with ab235446 at 1/500 (right) compared with a Isotype control details (ab172730) (left). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

TRP1 is predominantly expressed in mature melanosomes (PMID: 27073483, 11266471).
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR21956] (ab235446)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MeWo (human malignant melanoma cell line) cells labeling TRP1 with ab235446 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the MeWo cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-TRP1 antibody [EPR21956] (ab235446)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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