Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TRP1 antibody [EPR21956] (ab235446)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21956] to TRP1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TRP1 antibody [EPR21956]
    See all TRP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21956] to TRP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MeWo, SK-MEL-2 and SK-MEL-28 whole cell lysates. IHC-P: Human skin and melanoma tissue. ICC/IF: MeWo cells. Flow cyt: MeWo cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-TRP1 antibody [EPR21956] (ab235446)
    Western blot - Anti-TRP1 antibody [EPR21956] (ab235446)
    All lanes : Anti-TRP1 antibody [EPR21956] (ab235446) at 1/1000 dilution

    Lane 1 : MeWo (human malignant melanoma cell line) whole cell lysate
    Lane 2 : SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate
    Lane 3 : SK-MEL-28 (human malignant melanoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 8 seconds, Lanes 2 & 3: 3 minutes.

    The observed mass is greater than the predicted due to glycosylation.
    The molecular mass observed is consistent with what has been described in the literature (PMID: 21324755; PMID: 18042623; 18650849).
    The blot with lanes 2 and 3 was developed with a high sensitivity ECL substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21956] (ab235446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21956] (ab235446)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling TRP1 with ab235446 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of melanocytes in human skin (PMID:21562572) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21956] (ab235446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR21956] (ab235446)

    Immunohistochemical analysis of paraffin-embedded human melanoma tissue labeling TRP1 with ab235446 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining in tumor cells of human melanoma (PMID: 22045183; 26068396) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

     

  • Flow Cytometry - Anti-TRP1 antibody [EPR21956] (ab235446)
    Flow Cytometry - Anti-TRP1 antibody [EPR21956] (ab235446)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MeWo (human malignant melanoma cell line) cells labeling TRP1 with ab235446 at 1/500 (right) compared with a Isotype control details (ab172730) (left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    TRP1 is predominantly expressed in mature melanosomes (PMID: 27073483, 11266471).

  • Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR21956] (ab235446)
    Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR21956] (ab235446)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MeWo (human malignant melanoma cell line) cells labeling TRP1 with ab235446 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the MeWo cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Anti-TRP1 antibody [EPR21956] (ab235446)
    Anti-TRP1 antibody [EPR21956] (ab235446)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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