Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TRP1 antibody [EPR13063] (ab178676)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13063] to TRP1
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-TRP1 antibody [EPR13063]
See all TRP1 primary antibodies
Description

Rabbit monoclonal [EPR13063] to TRP1
Host species

Rabbit
Specificity

The mouse recommendation is based on the WB results. We do not guarantee IHC-P for mouse.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

within Human TRP1 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P17643
Positive control
- Human melanoma lysate; B16-F0 cell lysate; Human skin tissue; B16-F0 cells. WB: MeWo, B16-F0 and SK-MEL-28 cell lysates
General notes
This antibody is unsuitable for IHC-P on mouse samples.

Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13063
Isotype

IgG
Research areas

Flow Cytometry - Anti-TRP1 antibody [EPR13063] (ab178676)

Flow Cytometry analysis of B16-F0 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1:40 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)

All lanes : Anti-TRP1 antibody [EPR13063] (ab178676) at 1/50000 dilution (Purified)

Lane 1 : MeWo (Human malignant melanoma fibroblast) whole cell lysates
Lane 2 : B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

The observed molecular weight is consistent with what have been described in literatures PMID: 24586946 and 27690000.
Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)

Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution (Purified) + SK-MEL-28 (Human malignant melanoma ) whole cell lysates at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 61 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

The observed molecular weight is consistent with what have been described in literatures PMID: 24586946 and 27690000.
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)

Immunocytochemistry/ Immunofluorescence analysis of B16-F10 (Mouse melanoma epithelial cell-like) cells labeling TRP1 with purified ab178676 at 1:100 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling TRP1 with purified ab178676 at 1:1000 dilution (0.36 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)

Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution (unpurified) + Human melanoma lysate at 10 µg

Predicted band size: 61 kDa
Western blot - Anti-TRP1 antibody [EPR13063] (ab178676)

Anti-TRP1 antibody [EPR13063] (ab178676) at 1/10000 dilution (unpurified) + B16-F0 cell lysate at 10 µg

Predicted band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRP1 antibody [EPR13063] (ab178676)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TRP1 antibody [EPR13063] (ab178676)

Immunofluorescent analysis of B16-F0 cells labeling TRP1 with ab178676 (unpurified) at 1/100 dilution.
Anti-TRP1 antibody [EPR13063] (ab178676)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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