All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab228925) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

Lysates/proteins at 30 µg per lane.

Predicted band size: 26 kDa

12% SDS-PAGE gel.

Paraffin-embedded mouse fore brain tissue stained for Proteasome 20S alpha 5/PSMA5 using ab228925 at 1/500 dilution in immunohistochemical analysis.

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Proteasome 20S alpha 5/PSMA5 (green) using ab228925 at 1/500 dilution in ICC/IF.

Blue: Hoechst 33342 staining.

All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab228925) at 1/1000 dilution

Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

Lysates/proteins at 30 µg per lane.

Predicted band size: 26 kDa

12% SDS-PAGE gel.