Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
Key features and details
- Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 20S alpha 5/PSMA5 antibody
See all Proteasome 20S alpha 5/PSMA5 primary antibodies -
Description
Rabbit polyclonal to Proteasome 20S alpha 5/PSMA5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Zebrafish
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Immunogen
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General notes
This product was previously labelled as Proteasome 20S alpha 5
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)
Lane 1 : HeLa cell extract.
Lane 2 : HeLa cell extract with blocking peptide.
Predicted band size: 26.5 kDa
Observed band size: 26.5 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)Immunofluorescence of HeLa cells using ab11437. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody (ab11437)IHC image of ab11437 staining in human liver carcinoma liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11437, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
