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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)

Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5833] to Proteasome 20S alpha 5/PSMA5
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833]
    See all Proteasome 20S alpha 5/PSMA5 primary antibodies
  • Description

    Rabbit monoclonal [EPR5833] to Proteasome 20S alpha 5/PSMA5
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, Hela, 293T and JAR cell lysates; Human brain tissue.
  • General notes

     

     

     This product was previously labelled as Proteasome 20S alpha 5

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5833
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Images

  • Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    Western blot - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    All lanes : Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)

    ab109533, at 1/100 dilution, staining Proteasome 20S alpha 5/PSMA5 in paraffin embedded Human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)
    Anti-Proteasome 20S alpha 5/PSMA5 antibody [EPR5833] (ab109533)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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