Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSMA4 antibody [EPR5831(2)] (ab191403)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5831(2)] to PSMA4
  • Suitable for: ICC/IF, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PSMA4 antibody [EPR5831(2)]
    See all PSMA4 primary antibodies

  • Description

    Rabbit monoclonal [EPR5831(2)] to PSMA4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, K562, HeLa, Jar, rat C6, rat PC12 and mouse NIH 3T3 cell lysates. Human papillary adenocarcinoma of thyroid and mouse liver tissues. HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5831(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    Western blot - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    All lanes : Anti-PSMA4 antibody [EPR5831(2)] (ab191403) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : JAR cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Western blot - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    Western blot - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    All lanes : Anti-PSMA4 antibody [EPR5831(2)] (ab191403) at 1/1000 dilution

    Lane 1 : rat C6 cell lysate
    Lane 2 : mouse Raw 264.7 cell lysate
    Lane 3 : rat PC12 cell lysate
    Lane 4 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)

    Immunohistochemical analysis of paraffin-embedded, Human papillary adenocarcinoma of thyroid tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)

    Immunohistochemical analysis of paraffin-embedded, mouse liver tissue labeling PSMA4 with ab191403 at a 1/2000 dilution (1μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    Immunocytochemistry/ Immunofluorescence - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)

    Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling PSMA4 with ab191403 at a 1/500 dilution ( 4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-PSMA4 at a 1/500 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

  • Immunoprecipitation - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    Immunoprecipitation - Anti-PSMA4 antibody [EPR5831(2)] (ab191403)

    Western blot analysis on immunoprecipitation from 1) Jurkat cell lysate and 2) PBS, labeling PSMA4 using ab191403 at 1/200 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.

  • Anti-PSMA4 antibody [EPR5831(2)] (ab191403)
    Anti-PSMA4 antibody [EPR5831(2)] (ab191403)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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