Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ISG15 antibody [EPR3446] (ab133346)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3446] to ISG15
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ISG15 antibody [EPR3446]
    See all ISG15 primary antibodies

  • Description

    Rabbit monoclonal [EPR3446] to ISG15

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues of Human ISG15 (UniProt: P05161).

  • Positive control

    • WB: Daudi whole cell lysate (ab3951), IFN treated wild type HAP1 cell lysate, HeLa (untreated) and HeLa treated with 10 ng/ml IFN- a1 for 16 hours whole cell lysates ICC/IF: HeLa cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ISG15 antibody [EPR3446] (ab133346)
    Western blot - Anti-ISG15 antibody [EPR3446] (ab133346)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: HAP1 IFN treated wild type HAP1 whole cell lysate (20 µg)
    Lane 3: ISG15 (KO) whole cell lysate (20 µg)
    Lane 4: ISG15 (KO) IFN alpha treated whole cell lysate (20 µg)
    Lane 5: Daudi whole cell lysate (20 µg)
    Lanes 1 - 5: Merged signal (red and green). Green - ab133346 observed at 18 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab133346 was shown to specifically react with HAP1 IFN treated when HAP1 IFN treated knockout samples were used. Wild-type and HAP1 IFN treated knockout samples were subjected to SDS-PAGE. Ab133346 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/30000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ISG15 antibody [EPR3446] (ab133346)
    Western blot - Anti-ISG15 antibody [EPR3446] (ab133346)
    All lanes : Anti-ISG15 antibody [EPR3446] (ab133346) at 1/2000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10 ng/ml IFN- a1 for 16 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 18 kDa
    Observed band size: 17 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-ISG15 antibody [EPR3446] (ab133346)
    Immunocytochemistry/ Immunofluorescence - Anti-ISG15 antibody [EPR3446] (ab133346)
    ImmunImmunofluorescent analysis of ISG15 in HeLa cells stained with ab133346 at a 1/100 dilution.
  • Anti-ISG15 antibody [EPR3446] (ab133346)
    Anti-ISG15 antibody [EPR3446] (ab133346)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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