Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 130 665 ₸

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Anti-ISG15 antibody [EPR3446] (ab133346)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3446] to ISG15
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-ISG15 antibody [EPR3446]
See all ISG15 primary antibodies
Description

Rabbit monoclonal [EPR3446] to ISG15
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues of Human ISG15 (UniProt: P05161).
Positive control
- WB: Daudi whole cell lysate (ab3951), IFN treated wild type HAP1 cell lysate, HeLa (untreated) and HeLa treated with 10 ng/ml IFN- a1 for 16 hours whole cell lysates ICC/IF: HeLa cells
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3446
Isotype

IgG
Research areas

Western blot - Anti-ISG15 antibody [EPR3446] (ab133346)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: HAP1 IFN treated wild type HAP1 whole cell lysate (20 µg)
Lane 3: ISG15 (KO) whole cell lysate (20 µg)
Lane 4: ISG15 (KO) IFN alpha treated whole cell lysate (20 µg)
Lane 5: Daudi whole cell lysate (20 µg)
Lanes 1 - 5: Merged signal (red and green). Green - ab133346 observed at 18 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab133346 was shown to specifically react with HAP1 IFN treated when HAP1 IFN treated knockout samples were used. Wild-type and HAP1 IFN treated knockout samples were subjected to SDS-PAGE. Ab133346 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/30000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ISG15 antibody [EPR3446] (ab133346)

All lanes : Anti-ISG15 antibody [EPR3446] (ab133346) at 1/2000 dilution

Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10 ng/ml IFN- a1 for 16 hours whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 18 kDa
Observed band size: 17 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-ISG15 antibody [EPR3446] (ab133346)

ImmunImmunofluorescent analysis of ISG15 in HeLa cells stained with ab133346 at a 1/100 dilution.
Anti-ISG15 antibody [EPR3446] (ab133346)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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